pe cy7 labeled cd8a antibody  (Cytek Biosciences)

Journal: Pharmaceuticals

Article Title: Tamoxifen Protects from Vesicular Stomatitis Virus Infection

doi: 10.3390/ph12040142

Figure Lengend Snippet: TAM suppresses the VSV neutralizing antibody response. ( A ) VSV neutralizing antibodies were measured in sera harvested from TAM pretreated C57BL/6 mice (4 mg TAM i.p. per mouse, applied at day -3 and -1) and control mice (treated with corm oil) at the indicated time points after infection with 2 × 10 4 PFU VSV ( n = 6). The left graph shows the total amount of VSV neutralising antibodies measured without pretreatment with β-mercaptoethanol. The right graph shows the titer of VSV neutralising IgG antibodies in serum that was pretreated with β-mercaptoethanol to remove IgM and IgA antibodies. ( B ) Total amount of CD8+ T cells was analyzed in blood of TAM-pretreated and control mice 10 days after VSV infection by flow cytometry ( n = 6). ( C ) Percentage of CD8 + T cells from spleen capable to produce INF-γ after restimulation with virus-specific peptide p52 was measured in TAM pretreated and control mice 10 days after VSV infection by flow cytometry ( n = 6). Data are expressed as means ± SEM. n.s.: not significant, * p = 0.05; ** p = 0.01; *** p = 0.001; **** p < 0.001.

Article Snippet: CD8 positive cells were identified using PE-Cy7 labeled anti-CD8a antibody (eBioscience, Waltham, MA, USA).

Techniques: Control, Infection, Flow Cytometry, Virus